[Purposes] This paper aims to analyze the retraction trend of Chinese biomedical papers and the evolution of academic misconduct in biomedicine field in 2010-2019 and discuss the changes in recent years, thereby providing a basis and suggestions for targeted improvement of the research integrity system in this field in China. [Methods] The retracted Chinese biomedical papers from 2010 to 2019 were retrieved from Retraction Watch and the related retraction trend and evolution of academic misconduct in 2010-2019, 2010-2014, and 2015-2019 were respectively analyzed. [Findings] In 2010-2019, the retracted papers increased firstly and then decreased, while the retraction statements increased significantly and fluctuated at high levels since 2015. The retracted papers, retraction statements, and retracted papers in the same period in 2015-2019 were more than those in 2010-2014, and the duration from publication to retraction shortened significantly in 2015-2019. Since 2016, data problem and method and result problem have surpassed plagiarism and topped the academic misconduct behaviors. [Conclusions] The retraction trend of Chinese biomedical papers in 2015-2019 is more severe than that in 2010-2014 and the situation will remain grave in the future judging from the mainstream and constant deepened academic misconduct.