[Purposes] This study aims to survey the core readership of medical journals by questionnaire to understand the users' reading needs and reading experience of mobile terminals, and try to improve mobile terminal reading service in Journal of Third Military Medical University. [Methods] A total of 325 authors were selected from the manuscript online system of Journal of Third Military Medical University from April 1, 2015 to October 26, 2017, including the graduate students, scientific researchers, doctors, and teachers in medical colleges. A questionnaire survey was conducted from the basic situation, reading needs, reading behavior, and reading experience of the respondents in the form of network questionnaire (wjx.cn). [Findings] We collected 325 questionnaires, all of them are valid, and the valid recovery rate is 100%. The results show that 92.31% of 325 users are willing to use mobile terminals to read medical journals. Mobile reading is more popular among young and middle-aged readers at the age of 20-40 and doctors. Journal of Third Military Medical University is trying to cooperate with database platform service provider to carry out the mobile publishing service, to push the latest full papers, special reports, operation video, academic video live broadcast, and to push clinical research design lecture, science bulletin, and guide reading issued by WeChat official account to the core readers. [Conclusions] It is necessary for the medical journals to carry out the mobile terminal reading service. Most of medical journals published in single publication mode can enhance communication capacity, improve reading experience, and meet users' reading needs with the help of database platform providers and WeChat platform.